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pET30-ARH3-His-Sumo-HA
(Plasmid #111578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30
  • Modifications to backbone
    Tag insertions (see tags)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ARH3
  • Alt name
    Poly(ADP-ribose) glycohydrolase ARH3
  • Alt name
    ADPRHL2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1050
  • Mutation
    deleted amino acids 1-13
  • Entrez Gene
    ADPRS (a.k.a. ADPRHL2, ARH3, CONDSIAS)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis-Sumo (N terminal on backbone)
    • HA (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-ARH3-His-Sumo-HA was a gift from Thomas Muir (Addgene plasmid # 111578 ; http://n2t.net/addgene:111578 ; RRID:Addgene_111578)

  • For your References section:

    Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. Liszczak G, Diehl KL, Dann GP, Muir TW. Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 10.1038/s41589-018-0097-1. 10.1038/s41589-018-0097-1 PubMed 30013063
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