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PurposeExpresses full length HPF1 in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111577 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET30
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Modifications to backboneTag insertions (see tags)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHPF1
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Alt nameC4orf27
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Alt nameHistone PARylation factor 1
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SpeciesH. sapiens (human)
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Insert Size (bp)1038
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Entrez GeneHPF1 (a.k.a. C4orf27)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-Sumo-FLAG (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET30-HPF1-His-Sumo-Flag was a gift from Thomas Muir (Addgene plasmid # 111577 ; http://n2t.net/addgene:111577 ; RRID:Addgene_111577) -
For your References section:
Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. Liszczak G, Diehl KL, Dann GP, Muir TW. Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 10.1038/s41589-018-0097-1. 10.1038/s41589-018-0097-1 PubMed 30013063
