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Addgene

pACEBac1-PARP1-Flag-His-WT
(Plasmid #111572)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111572 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Modifications to backbone
    C-terminal Flag-6xHis
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PARP1
  • Alt name
    Poly(ADP-ribose) polymerase 1
  • Alt name
    ARTD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3042
  • Entrez Gene
    PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
  • Promoter polyhedrin promoter
  • Tags / Fusion Proteins
    • C-terminal 6xHis (C terminal on backbone)
    • C-terminal Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (on insert), BamHI (in vector) (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Custom (gtatcgattcgcgacctactcc)
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEBac1-PARP1-Flag-His-WT was a gift from Thomas Muir (Addgene plasmid # 111572 ; http://n2t.net/addgene:111572 ; RRID:Addgene_111572)

  • For your References section:

    Acetylation blocks DNA damage-induced chromatin ADP-ribosylation. Liszczak G, Diehl KL, Dann GP, Muir TW. Nat Chem Biol. 2018 Jul 16. pii: 10.1038/s41589-018-0097-1. doi: 10.1038/s41589-018-0097-1. 10.1038/s41589-018-0097-1 PubMed 30013063
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