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Addgene

pCabp5-DsRed
(Plasmid #11157)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11157 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCAGEN
  • Backbone size w/o insert (bp) 3084
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cabp5 promoter
  • Alt name
    Calcium binding protein 5 promoter
  • Alt name
    Cabp5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4679
  • Entrez Gene
    Cabp5
  • Tag / Fusion Protein
    • DsRed2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer DsRed-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Cabp5 promoter is specific for bipolar cells in the retina

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCabp5-DsRed was a gift from Connie Cepko (Addgene plasmid # 11157 ; http://n2t.net/addgene:11157 ; RRID:Addgene_11157)
  • For your References section:

    Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031