pZac2.1-GfaABC1D-mCherry-hPMCA2x/b
(Plasmid #111569)

• Purpose: Expresses a plasma membrane calcium pump hPMCA2x/b
• Depositing Lab: Baljit Khakh
• Publication: Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)30688-3. doi: 10.1016/j.neuron.2018.08.015.

Backbone

• Vector backbone: pZac2.1
• Backbone size w/o insert (bp): 4454
• Total vector size (bp): 8936

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Plasma membrane calcium pump 2, variant x/b
• Alt name: PMCA2x/b
• Species: H. sapiens (human)
• Insert Size (bp): 3639
• GenBank ID: NM_001036684.2
• Entrez Gene: ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Resource Information

• Supplemental Documents:
  • pZac2.1-GfaABC1D-mCherry-hPMCA2xb Addgene.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This sequence for PMCA2x/b contains a G73D mutation compared to the reference but the depositing lab confirms that this mutation does not affect plasmid function.