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Agarabi CHO Light Chain in pJ201
(Plasmid #111566)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ201
  • Backbone manufacturer
    ATUM (formerly DNA 2.0)
  • Backbone size w/o insert (bp) 2673
  • Total vector size (bp) 3389
  • Vector type
    Synthetic Biology ; Cloning Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Culture transformants in LB broth supplemented with 50 ug/mL Kanamycin
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Murine light chain
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    716
  • GenBank ID
    AKH40269.1
  • Promoter None

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Agarabi CHO Light Chain in pJ201 was a gift from Cyrus Agarabi (Addgene plasmid # 111566 ; http://n2t.net/addgene:111566 ; RRID:Addgene_111566)

  • For your References section:

    Impact of media and antifoam selection on monoclonal antibody production and quality using a high throughput micro-bioreactor system. Velugula-Yellela SR, Williams A, Trunfio N, Hsu CJ, Chavez B, Yoon S, Agarabi C. Biotechnol Prog. 2018 Jan;34(1):262-270. doi: 10.1002/btpr.2575. Epub 2017 Nov 16. 10.1002/btpr.2575 PubMed 29086492
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