Skip to main content
Addgene

pCAG-mGSDMD1-251
(Plasmid #111565)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111565 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCAG-FOS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gasdermin D
  • Alt name
    GSDMD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    753
  • Entrez Gene
    Gsdmd (a.k.a. 1810036L03Rik, DF5L, Dfna5l, GsdmD-1, Gsdmdc1, M2-4)
  • Promoter CAG
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tcaaacgcgtCTCGAGcaccATGCCATCGGCCTTTGAGAAAGTGG
  • 3′ sequencing primer tagcaagcttCTCGAGcactgcttttctgtcacctgaggag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Backbone is from Dr. Eiji Morita

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mGSDMD1-251 was a gift from Hongbo Luo (Addgene plasmid # 111565 ; http://n2t.net/addgene:111565 ; RRID:Addgene_111565)

  • For your References section:

    Gasdermin D Exerts Anti-inflammatory Effects by Promoting Neutrophil Death. Kambara H, Liu F, Zhang X, Liu P, Bajrami B, Teng Y, Zhao L, Zhou S, Yu H, Zhou W, Silberstein LE, Cheng T, Han M, Xu Y, Luo HR. Cell Rep. 2018 Mar 13;22(11):2924-2936. doi: 10.1016/j.celrep.2018.02.067. 10.1016/j.celrep.2018.02.067 PubMed 29539421
Related items:
Commonly requested with: