pRho-DsRed
(Plasmid #11156)

• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 3086
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rhodopsin promoter
• Alt name: Bos taurus Rho
• Alt name: Rho promoter
• Species: B. taurus (bovine)
• Insert Size (bp): 2239
• Entrez Gene: RHO
• Tag / Fusion Protein:
  • DsRed2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: DsRed-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The bovine rhodopsin promoter was from -2174-lacZ (Zack et al., Neuron 6, 187-199 (1991)) obtained from Dr. D. Zack (Johns Hopkins University).
• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The rhodopsin promoter is specific for photoreceptor cells in the retina.