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Addgene

Thy-C73A-HA
(Plasmid #111542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111542 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    thy1.2-C73A-HA
  • Alt name
    KCNB1
  • Species
    H. sapiens (human)
  • Mutation
    C73A
  • Entrez Gene
    KCNB1 (a.k.a. DRK1, Kv2.1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Thy-C73A-HA was a gift from Federico Sesti (Addgene plasmid # 111542 ; http://n2t.net/addgene:111542 ; RRID:Addgene_111542)

  • For your References section:

    Oxidation of KCNB1 Potassium Channels Causes Neurotoxicity and Cognitive Impairment in a Mouse Model of Traumatic Brain Injury. Yu W, Parakramaweera R, Teng S, Gowda M, Sharad Y, Thakker-Varia S, Alder J, Sesti F. J Neurosci. 2016 Oct 26;36(43):11084-11096. doi: 10.1523/JNEUROSCI.2273-16.2016. 10.1523/JNEUROSCI.2273-16.2016 PubMed 27798188

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