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hC73A-pEGFP_N1
(Plasmid #111533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human C73A-Kv2.1
  • Alt name
    KCNB1
  • Species
    H. sapiens (human)
  • Mutation
    C73A
  • Entrez Gene
    KCNB1 (a.k.a. DRK1, Kv2.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hC73A-pEGFP_N1 was a gift from Federico Sesti (Addgene plasmid # 111533 ; http://n2t.net/addgene:111533 ; RRID:Addgene_111533)

  • For your References section:

    Oxidation of KCNB1 potassium channels triggers apoptotic integrin signaling in the brain. Yu W, Gowda M, Sharad Y, Singh SA, Sesti F. Cell Death Dis. 2017 Apr 6;8(4):e2737. doi: 10.1038/cddis.2017.160. 10.1038/cddis.2017.160 PubMed 28383553
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