pCMV-GFP Citations (63)
Originally described in: Electroporation and RNA interference in the rodent retina in vivo and in vitro.Matsuda T, Cepko CL Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. PubMed Journal
Articles Citing pCMV-GFP
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JAK/STAT3 pathway inhibition blocks skeletal muscle wasting downstream of IL-6 and in experimental cancer cachexia. Bonetto A, Aydogdu T, Jin X, Zhang Z, Zhan R, Puzis L, Koniaris LG, Zimmers TA. Am J Physiol Endocrinol Metab. 2012 Aug 1;303(3):E410-21. doi: 10.1152/ajpendo.00039.2012. Epub 2012 Jun 5. PubMed |
Efficient transfection of DNA into primarily cultured rat sertoli cells by electroporation. Li F, Yamaguchi K, Okada K, Matsushita K, Enatsu N, Chiba K, Yue H, Fujisawa M. Biol Reprod. 2013 Mar 14;88(3):61. doi: 10.1095/biolreprod.112.106260. Print 2013 Mar. PubMed |
An adult zebrafish model for preclinical tuberculosis vaccine development. Oksanen KE, Halfpenny NJ, Sherwood E, Harjula SK, Hammaren MM, Ahava MJ, Pajula ET, Lahtinen MJ, Parikka M, Ramet M. Vaccine. 2013 Oct 25;31(45):5202-9. doi: 10.1016/j.vaccine.2013.08.093. Epub 2013 Sep 20. PubMed |
Analysis of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. Aschauer DF, Kreuz S, Rumpel S. PLoS One. 2013 Sep 27;8(9):e76310. doi: 10.1371/journal.pone.0076310. eCollection 2013. PubMed |
microRNAs miR-27a and miR-27b directly regulate liver dihydropyrimidine dehydrogenase expression through two conserved binding sites. Offer SM, Butterfield GL, Jerde CR, Fossum CC, Wegner NJ, Diasio RB. Mol Cancer Ther. 2014 Mar;13(3):742-51. doi: 10.1158/1535-7163.MCT-13-0878. Epub 2014 Jan 8. PubMed |
Mannosylated poly(beta-amino esters) for targeted antigen presenting cell immune modulation. Jones CH, Chen M, Ravikrishnan A, Reddinger R, Zhang G, Hakansson AP, Pfeifer BA. Biomaterials. 2015 Jan;37:333-44. doi: 10.1016/j.biomaterials.2014.10.037. Epub 2014 Oct 22. PubMed |
Genetic screening reveals a link between Wnt signaling and antitubulin drugs. Khan AH, Bloom JS, Faridmoayer E, Smith DJ. Pharmacogenomics J. 2015 Jul 7. doi: 10.1038/tpj.2015.50. PubMed |
DNA vaccination boosts Bacillus Calmette-Guerin protection against mycobacterial infection in zebrafish. Oksanen KE, Myllymaki H, Ahava MJ, Makinen L, Parikka M, Ramet M. Dev Comp Immunol. 2016 Jan;54(1):89-96. doi: 10.1016/j.dci.2015.09.001. Epub 2015 Sep 9. PubMed |
In vivo dynamics of skeletal muscle Dystrophin in zebrafish embryos revealed by improved FRAP analysis. Bajanca F, Gonzalez-Perez V, Gillespie SJ, Beley C, Garcia L, Theveneau E, Sear RP, Hughes SM. Elife. 2015 Oct 13;4. doi: 10.7554/eLife.06541. PubMed |
Stage-specific differentiation of iPSCs toward retinal ganglion cell lineage. Deng F, Chen M, Liu Y, Hu H, Xiong Y, Xu C, Liu Y, Li K, Zhuang J, Ge J. Mol Vis. 2016 May 28;22:536-47. eCollection 2016. PubMed |
Yap reprograms glutamine metabolism to increase nucleotide biosynthesis and enable liver growth. Cox AG, Hwang KL, Brown KK, Evason KJ, Beltz S, Tsomides A, O'Connor K, Galli GG, Yimlamai D, Chhangawala S, Yuan M, Lien EC, Wucherpfennig J, Nissim S, Minami A, Cohen DE, Camargo FD, Asara JM, Houvras Y, Stainier DY, Goessling W. Nat Cell Biol. 2016 Aug;18(8):886-96. doi: 10.1038/ncb3389. Epub 2016 Jul 18. PubMed |
Cross talk among PMCA, calcineurin and NFAT transcription factors in control of calmodulin gene expression in differentiating PC12 cells. Boczek T, Lisek M, Ferenc B, Zylinska L. Biochim Biophys Acta. 2017 Apr;1860(4):502-515. doi: 10.1016/j.bbagrm.2017.01.012. Epub 2017 Jan 31. PubMed |
Long-Term Two-Photon Imaging in Awake Macaque Monkey. Li M, Liu F, Jiang H, Lee TS, Tang S. Neuron. 2017 Mar 8;93(5):1049-1057.e3. doi: 10.1016/j.neuron.2017.01.027. Epub 2017 Feb 16. PubMed |
Widespread Influence of 3'-End Structures on Mammalian mRNA Processing and Stability.
Wu X, Bartel DP.
Cell. 2017 May 18;169(5):905-917.e11. doi: 10.1016/j.cell.2017.04.036.
PubMed
Associated Plasmids |
Identification of novel antigen candidates for a tuberculosis vaccine in the adult zebrafish (Danio rerio). Myllymaki H, Niskanen M, Oksanen KE, Sherwood E, Ahava M, Parikka M, Ramet M. PLoS One. 2017 Jul 25;12(7):e0181942. doi: 10.1371/journal.pone.0181942. eCollection 2017. PubMed |
A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer. Merenda A, Andersson-Rolf A, Mustata RC, Li T, Kim H, Koo BK. J Vis Exp. 2017 Jul 12;(125). doi: 10.3791/55916. PubMed |
Effective scheme of photolysis of GFP in live cell as revealed with confocal fluorescence microscopy. Glazachev YI, Orlova DY, Reznickova P, Bartova E. Phys Biol. 2018 Mar 23;15(3):036008. doi: 10.1088/1478-3975/aab31e. PubMed |
Wnt3 and Gata4 regulate axon regeneration in adult mouse DRG neurons. Duan RS, Liu PP, Xi F, Wang WH, Tang GB, Wang RY, Saijilafu, Liu CM. Biochem Biophys Res Commun. 2018 May 5;499(2):246-252. doi: 10.1016/j.bbrc.2018.03.138. Epub 2018 Mar 24. PubMed |
Identification of protective postexposure mycobacterial vaccine antigens using an immunosuppression-based reactivation model in the zebrafish. Myllymaki H, Niskanen M, Luukinen H, Parikka M, Ramet M. Dis Model Mech. 2018 Mar 13;11(3). pii: 11/3/dmm033175. doi: 10.1242/dmm.033175. PubMed |
Regulation of miR-34b/c-targeted gene expression program by SUMOylation. Li YJ, Du L, Aldana-Masangkay G, Wang X, Urak R, Forman SJ, Rosen ST, Chen Y. Nucleic Acids Res. 2018 Aug 21;46(14):7108-7123. doi: 10.1093/nar/gky484. PubMed |
Live-cell microscopy of meiosis in spermatocytes. Shibuya H, Watanabe Y. Methods Cell Biol. 2018;145:269-277. doi: 10.1016/bs.mcb.2018.03.028. Epub 2018 Apr 19. PubMed |
Lin28 Signaling Supports Mammalian PNS and CNS Axon Regeneration. Wang XW, Li Q, Liu CM, Hall PA, Jiang JJ, Katchis CD, Kang S, Dong BC, Li S, Zhou FQ. Cell Rep. 2018 Sep 4;24(10):2540-2552.e6. doi: 10.1016/j.celrep.2018.07.105. PubMed |
Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines. Myllymaki H, Niskanen M, Oksanen K, Ramet M. J Vis Exp. 2018 Oct 30;(140). doi: 10.3791/58453. PubMed |
Regulation of cell-type-specific transcriptomes by microRNA networks during human brain development. Nowakowski TJ, Rani N, Golkaram M, Zhou HR, Alvarado B, Huch K, West JA, Leyrat A, Pollen AA, Kriegstein AR, Petzold LR, Kosik KS. Nat Neurosci. 2018 Dec;21(12):1784-1792. doi: 10.1038/s41593-018-0265-3. Epub 2018 Nov 19. PubMed |
The Pat1-Lsm Complex Stabilizes ATG mRNA during Nitrogen Starvation-Induced Autophagy.
Gatica D, Hu G, Liu X, Zhang N, Williamson PR, Klionsky DJ.
Mol Cell. 2019 Jan 17;73(2):314-324.e4. doi: 10.1016/j.molcel.2018.11.002. Epub 2018 Dec 6.
PubMed
Associated Plasmids |
Nucleolin Is a Functional Binding Protein for Salinomycin in Neuroblastoma Stem Cells. Wang F, Zhou S, Qi D, Xiang SH, Wong ET, Wang X, Fonkem E, Hsieh TC, Yang J, Kirmani B, Shabb JB, Wu JM, Wu M, Huang JH, Yu WH, Wu E. J Am Chem Soc. 2019 Feb 27;141(8):3613-3622. doi: 10.1021/jacs.8b12872. Epub 2019 Feb 13. PubMed |
Differential roles of epigenetic regulators in the survival and differentiation of oligodendrocyte precursor cells. Egawa N, Shindo A, Hikawa R, Kinoshita H, Liang AC, Itoh K, Lok J, Maki T, Takahashi R, Lo EH, Arai K. Glia. 2019 Apr;67(4):718-728. doi: 10.1002/glia.23567. Epub 2018 Nov 28. PubMed |
Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement. Teng LL, Lu GL, Chiou LC, Lin WS, Cheng YY, Hsueh TE, Huang YC, Hwang NH, Yeh JW, Liao RM, Fan SZ, Yen JH, Fu TF, Tsai TF, Wu MS, Wang PY. PLoS Biol. 2019 Mar 18;17(3):e2007097. doi: 10.1371/journal.pbio.2007097. eCollection 2019 Mar. PubMed |
Deoxyuridine in DNA has an inhibitory and promutagenic effect on RNA transcription by diverse RNA polymerases. Cui J, Gizzi A, Stivers JT. Nucleic Acids Res. 2019 May 7;47(8):4153-4168. doi: 10.1093/nar/gkz183. PubMed |
The role of ORMDL3/ATF6 in compensated beta cell proliferation during early diabetes. Yang W, Sheng F, Sun B, Fischbach S, Xiao X. Aging (Albany NY). 2019 May 6;11(9):2787-2796. doi: 10.18632/aging.101949. PubMed |
Radiation-induced glucocorticoid receptor promotes CD44+ prostate cancer stem cell growth through activation of SGK1-Wnt/beta-catenin signaling. Chen F, Chen X, Ren Y, Weng G, Keng PC, Chen Y, Lee SO. J Mol Med (Berl). 2019 Aug;97(8):1169-1182. doi: 10.1007/s00109-019-01807-8. Epub 2019 Jun 11. PubMed |
Pyruvate kinase M2 is requisite for Th1 and Th17 differentiation. Kono M, Maeda K, Stocton-Gavanescu I, Pan W, Umeda M, Katsuyama E, Burbano C, Orite SYK, Vukelic M, Tsokos MG, Yoshida N, Tsokos GC. JCI Insight. 2019 Jun 20;4(12). pii: 127395. doi: 10.1172/jci.insight.127395. eCollection 2019 Jun 20. PubMed |
Non-viral Gene Therapy for Stargardt Disease with ECO/pRHO-ABCA4 Self-Assembled Nanoparticles. Sun D, Schur RM, Sears AE, Gao SQ, Vaidya A, Sun W, Maeda A, Kern T, Palczewski K, Lu ZR. Mol Ther. 2020 Jan 8;28(1):293-303. doi: 10.1016/j.ymthe.2019.09.010. Epub 2019 Sep 12. PubMed |
Cell-free transcription in Xenopus egg extract. Barrows JK, Long DT. J Biol Chem. 2019 Dec 20;294(51):19645-19654. doi: 10.1074/jbc.RA119.011350. Epub 2019 Nov 15. PubMed |
Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells. Belling JN, Heidenreich LK, Tian Z, Mendoza AM, Chiou TT, Gong Y, Chen NY, Young TD, Wattanatorn N, Park JH, Scarabelli L, Chiang N, Takahashi J, Young SG, Stieg AZ, De Oliveira S, Huang TJ, Weiss PS, Jonas SJ. Proc Natl Acad Sci U S A. 2020 May 19;117(20):10976-10982. doi: 10.1073/pnas.1917125117. Epub 2020 May 1. PubMed |
Oncogenic hijacking of a developmental transcription factor evokes vulnerability toward oxidative stress in Ewing sarcoma. Marchetto A, Ohmura S, Orth MF, Knott MML, Colombo MV, Arrigoni C, Bardinet V, Saucier D, Wehweck FS, Li J, Stein S, Gerke JS, Baldauf MC, Musa J, Dallmayer M, Romero-Perez L, Holting TLB, Amatruda JF, Cossarizza A, Henssen AG, Kirchner T, Moretti M, Cidre-Aranaz F, Sannino G, Grunewald TGP. Nat Commun. 2020 May 15;11(1):2423. doi: 10.1038/s41467-020-16244-2. PubMed |
DNA vaccination with the Mycobacterium marinum MMAR_4110 antigen inhibits reactivation of a latent mycobacterial infection in the adult Zebrafish. Niskanen M, Myllymaki H, Ramet M. Vaccine. 2020 Jul 31;38(35):5685-5694. doi: 10.1016/j.vaccine.2020.06.053. Epub 2020 Jul 3. PubMed |
In Vitro Evaluation of Lipopolyplexes for Gene Transfection: Comparing 2D, 3D and Microdroplet-Enabled Cell Culture. Paris JL, Coelho F, Teixeira A, Dieguez L, Silva BFB, Abalde-Cela S. Molecules. 2020 Jul 18;25(14). pii: molecules25143277. doi: 10.3390/molecules25143277. PubMed |
Engineering carotenoid production in mammalian cells for nutritionally enhanced cell-cultured foods. Stout AJ, Mirliani AB, Soule-Albridge EL, Cohen JM, Kaplan DL. Metab Eng. 2020 Nov;62:126-137. doi: 10.1016/j.ymben.2020.07.011. Epub 2020 Sep 2. PubMed |
Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting. Brocker CN, Kim D, Melia T, Karri K, Velenosi TJ, Takahashi S, Aibara D, Bonzo JA, Levi M, Waxman DJ, Gonzalez FJ. Nat Commun. 2020 Nov 17;11(1):5847. doi: 10.1038/s41467-020-19554-7. PubMed |
Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs. Wang Y, Bi D, Qin G, Song R, Yao J, Cao C, Zheng Q, Hou N, Wang Y, Zhao J. Front Genet. 2020 Nov 16;11:592623. doi: 10.3389/fgene.2020.592623. eCollection 2020. PubMed |
BRCA1-BARD1 regulates transcription through BRD4 in Xenopus nucleoplasmic extract. Barrows JK, Fullbright G, Long DT. Nucleic Acids Res. 2021 Apr 6;49(6):3263-3273. doi: 10.1093/nar/gkab111. PubMed |
Noncontact Multiphysics Probe for Spatiotemporal Resolved Single-Cell Manipulation and Analyses. Brimmo AT, Menachery A, Sukumar P, Qasaimeh MA. Small. 2021 Jun;17(24):e2100801. doi: 10.1002/smll.202100801. Epub 2021 May 19. PubMed |
Nicotinamide riboside kinase-2 inhibits JNK pathway and limits dilated cardiomyopathy in mice with chronic pressure overload. Shahzadi SK, Marzook H, Qaisar R, Ahmad F. Clin Sci (Lond). 2022 Jan 28;136(2):181-196. doi: 10.1042/CS20210964. PubMed |
Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration. LaForce GR, Farr JS, Liu J, Akesson C, Gumus E, Pinkard O, Miranda HC, Johnson K, Sweet TJ, Ji P, Lin A, Coller J, Philippidou P, Wagner EJ, Schaffer AE. Neuron. 2022 Apr 20;110(8):1340-1357.e7. doi: 10.1016/j.neuron.2022.01.018. Epub 2022 Feb 8. PubMed |
Transiently expressed CRISPR/Cas9 induces wild-type dystrophin in vitro in DMD patient myoblasts carrying duplications. Pini V, Mariot V, Dumonceaux J, Counsell J, O'Neill HC, Farmer S, Conti F, Muntoni F. Sci Rep. 2022 Mar 8;12(1):3756. doi: 10.1038/s41598-022-07671-w. PubMed |
Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma. Chiu HY, Loh AHP, Taneja R. Cell Death Dis. 2022 Apr 30;13(4):419. doi: 10.1038/s41419-022-04835-4. PubMed |
Genetic and compound screens uncover factors modulating cancer cell response to indisulam. Pogacar Z, Groot K, Jochems F, Dos Santos Dias M, Mulero-Sanchez A, Morris B, Roosen M, Wardak L, De Conti G, Velds A, Lieftink C, Thijssen B, Beijersbergen RL, Bernards R, Leite de Oliveira R. Life Sci Alliance. 2022 May 9;5(9):e202101348. doi: 10.26508/lsa.202101348. Print 2022 Sep. PubMed |
BRD4 promotes resection and homology-directed repair of DNA double-strand breaks. Barrows JK, Lin B, Quaas CE, Fullbright G, Wallace EN, Long DT. Nat Commun. 2022 May 31;13(1):3016. doi: 10.1038/s41467-022-30787-6. PubMed |
Autophagy-associated immune dysregulation and hyperplasia in a patient with compound heterozygous mutations in ATG9A. Hu G, Hauk PJ, Zhang N, Elsegeiny W, Guardia CM, Kullas A, Crosby K, Deterding RR, Schedel M, Reynolds P, Abbott JK, Knight V, Pittaluga S, Raffeld M, Rosenzweig SD, Bonifacino JS, Uzel G, Williamson PR, Gelfand EW. Autophagy. 2023 Feb;19(2):678-691. doi: 10.1080/15548627.2022.2093028. Epub 2022 Jul 15. PubMed |
Indisulam synergizes with palbociclib to induce senescence through inhibition of CDK2 kinase activity. Pogacar Z, Johnson JL, Krenning L, De Conti G, Jochems F, Lieftink C, Velds A, Wardak L, Groot K, Schepers A, Wang L, Song JY, van de Ven M, van Tellingen O, Medema RH, Beijersbergen RL, Bernards R, Leite de Oliveira R. PLoS One. 2022 Sep 6;17(9):e0273182. doi: 10.1371/journal.pone.0273182. eCollection 2022. PubMed |
Effective gene therapy of Stargardt disease with PEG-ECO/pGRK1-ABCA4-S/MAR nanoparticles. Sun D, Sun W, Gao SQ, Lehrer J, Naderi A, Wei C, Lee S, Schilb AL, Scheidt J, Hall RC, Traboulsi EI, Palczewski K, Lu ZR. Mol Ther Nucleic Acids. 2022 Aug 24;29:823-835. doi: 10.1016/j.omtn.2022.08.026. eCollection 2022 Sep 13. PubMed |
Sox13 is a novel flow-sensitive transcription factor that prevents inflammation by repressing chemokine expression in endothelial cells. Demos C, Johnson J, Andueza A, Park C, Kim Y, Villa-Roel N, Kang DW, Kumar S, Jo H. Front Cardiovasc Med. 2022 Sep 30;9:979745. doi: 10.3389/fcvm.2022.979745. eCollection 2022. PubMed |
Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair. Liu L, Lin B, Yin S, Ball LE, Delaney JR, Long DT, Gan W. Sci Adv. 2022 Dec 9;8(49):eadd8928. doi: 10.1126/sciadv.add8928. Epub 2022 Dec 7. PubMed |
Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM).
Sarfraz N, Moscoso E, Oertel T, Lee HJ, Ranjit S, Braselmann E.
Nat Commun. 2023 Feb 16;14(1):867. doi: 10.1038/s41467-023-36531-y.
PubMed
Associated Plasmids |
A human proteogenomic-cellular framework identifies KIF5A as a modulator of astrocyte process integrity with relevance to ALS. Szebenyi K, Barrio-Hernandez I, Gibbons GM, Biasetti L, Troakes C, Beltrao P, Lakatos A. Commun Biol. 2023 Jun 29;6(1):678. doi: 10.1038/s42003-023-05041-4. PubMed |
Stability Criterion for the Assembly of Core-Shell Lipid-Polymer-Nucleic Acid Nanoparticles. Paris JL, Gaspar R, Coelho F, De Beule PAA, Silva BFB. ACS Nano. 2023 Sep 12;17(17):17587-17594. doi: 10.1021/acsnano.3c07204. Epub 2023 Aug 15. PubMed |
Tissue-specific in vivo transformation of plasmid DNA in Neotropical tadpoles using electroporation. Delia J, Gaines-Richardson M, Ludington SC, Akbari N, Vasek C, Shaykevich D, O'Connell LA. PLoS One. 2023 Aug 17;18(8):e0289361. doi: 10.1371/journal.pone.0289361. eCollection 2023. PubMed |
Fluorinated Silane-Modified Filtroporation Devices Enable Gene Knockout in Human Hematopoietic Stem and Progenitor Cells. Frost IM, Mendoza AM, Chiou TT, Kim P, Aizenberg J, Kohn DB, De Oliveira SN, Weiss PS, Jonas SJ. ACS Appl Mater Interfaces. 2023 Sep 6;15(35):41299-41309. doi: 10.1021/acsami.3c07045. Epub 2023 Aug 24. PubMed |
The substrate and inhibitor binding mechanism of polyspecific transporter OAT1 revealed by high-resolution cryo-EM. Dou T, Lian T, Shu S, He Y, Jiang J. Nat Struct Mol Biol. 2023 Nov;30(11):1794-1805. doi: 10.1038/s41594-023-01123-3. Epub 2023 Oct 16. PubMed |
Human paraneoplastic antigen Ma2 (PNMA2) forms icosahedral capsids that can be engineered for mRNA delivery. Madigan V, Zhang Y, Raghavan R, Wilkinson ME, Faure G, Puccio E, Segel M, Lash B, Macrae RK, Zhang F. Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2307812120. doi: 10.1073/pnas.2307812120. Epub 2024 Mar 4. PubMed |
Protocol for HiBiT tagging endogenous proteins using CRISPR-Cas9 gene editing. Lankford KP, Hulleman JD. STAR Protoc. 2024 Jun 21;5(2):103000. doi: 10.1016/j.xpro.2024.103000. Epub 2024 Apr 9. PubMed |
Emx2 underlies the development and evolution of marsupial gliding membranes. Moreno JA, Dudchenko O, Feigin CY, Mereby SA, Chen Z, Ramos R, Almet AA, Sen H, Brack BJ, Johnson MR, Li S, Wang W, Gaska JM, Ploss A, Weisz D, Omer AD, Yao W, Colaric Z, Kaur P, Leger JS, Nie Q, Mena A, Flanagan JP, Keller G, Sanger T, Ostrow B, Plikus MV, Kvon EZ, Aiden EL, Mallarino R. Nature. 2024 May;629(8010):127-135. doi: 10.1038/s41586-024-07305-3. Epub 2024 Apr 24. PubMed |
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