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Addgene

hC73S-HA
(Plasmid #111529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human C73S-Kv2.1 + HA
  • Alt name
    KCNB1
  • Species
    H. sapiens (human)
  • Mutation
    C73S
  • Entrez Gene
    KCNB1 (a.k.a. DRK1, Kv2.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hC73S-HA was a gift from Federico Sesti (Addgene plasmid # 111529 ; http://n2t.net/addgene:111529 ; RRID:Addgene_111529)

  • For your References section:

    Toxic role of K+ channel oxidation in mammalian brain. Cotella D, Hernandez-Enriquez B, Wu X, Li R, Pan Z, Leveille J, Link CD, Oddo S, Sesti F. J Neurosci. 2012 Mar 21;32(12):4133-44. doi: 10.1523/JNEUROSCI.6153-11.2012. 10.1523/JNEUROSCI.6153-11.2012 PubMed 22442077
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