hKv2.1-HA
(Plasmid #111525)

• Purpose: Expresses human WT KCNB1 in mammalian cells
• Depositing Lab: Federico Sesti
• Publication: Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44. doi: 10.1523/JNEUROSCI.6153-11.2012.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Kv2.1 + HA
• Alt name: KCNB1
• Species: H. sapiens (human)
• Entrez Gene: KCNB1 (a.k.a. DRK1, Kv2.1)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry