pCAG-HcRed
(Plasmid #11152)

• Purpose: Mammalian expression vector for expression of HcRed
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGEN
• Backbone manufacturer: Available at Addgene (#11160)
• Backbone size w/o insert (bp): 4779
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HcRed1 from Heteractis crispa
• Alt name: HcRed
• Alt name: far-red fluorescent protein
• Insert Size (bp): 741

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: HcRed1 was from pHcRed1-N1 (Clontech).
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CAG promoter (chicken beta-actin promoter with CMV enhancer) is more efficient than CMV promoter, see "Efficient selection for high-expression transfectants with a novel eukaryotic vector", Gene. 1991 Dec 15;108(2):193-9.