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pET21a - Ec-ClpX D144L
(Plasmid #111514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6736
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ClpX
  • Alt name
    ATP-dependent Clp protease ATP-binding subunit clpX
  • Species
    Escherichia coli
  • Insert Size (bp)
    1275
  • Mutation
    Aspartatic acid 144 to Leucine
  • GenBank ID
    NC_000913.3
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor confirms K408E compared to NC_000913.3 has no impact on function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a - Ec-ClpX D144L was a gift from Judy Lieberman (Addgene plasmid # 111514 ; http://n2t.net/addgene:111514 ; RRID:Addgene_111514)

  • For your References section:

    Granzyme B Disrupts Central Metabolism and Protein Synthesis in Bacteria to Promote an Immune Cell Death Program. Dotiwala F, Sen Santara S, Binker-Cosen AA, Li B, Chandrasekaran S, Lieberman J. Cell. 2017 Nov 16;171(5):1125-1137.e11. doi: 10.1016/j.cell.2017.10.004. Epub 2017 Oct 26. 10.1016/j.cell.2017.10.004 PubMed 29107333
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