pCAG-DsRed
(Plasmid #11151)

• Purpose: Mammalian expression vector for expression of DsRed
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGEN
• Backbone manufacturer: Available at Addgene (#11160)
• Backbone size w/o insert (bp): 4779
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DsRed2 from Discosoma sp
• Alt name: DsRed
• Alt name: red fluorescent protein
• Insert Size (bp): 728

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: DsRed-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: DsRed2 was from pDsRed2-N1 (Clontech).
• Articles Citing this Plasmid:
  • 27 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

CAG promoter (chicken beta-actin promoter with CMV enhancer) is more efficient than CMV promoter, see "Efficient selection for high-expression transfectants with a novel eukaryotic vector", Gene. 1991 Dec 15;108(2):193-9.

Please note that though there are some discrepancies between the assembled sequence from the depositing lab and Addgene's quality control sequence, this plasmid should function as reported.

Information for Cloning Grade DNA (Catalog # 11151-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here