pCAG-GFP
(Plasmid #11150)

• Purpose: Mammalian expression vector for expression of GFP (CAG promoter)
• Depositing Lab: Connie Cepko
• Publication: Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22.

Backbone

• Vector backbone: pCAGEN
• Backbone manufacturer: Available at Addgene (#11160)
• Backbone size w/o insert (bp): 4779

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP from Aequorea victoria
• Alt name: GFP
• Alt name: green fluorescent protein
• Insert Size (bp): 772

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EGFP was from pEGFP-N1 (Clontech).
• Articles Citing this Plasmid:
  • 174 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Addgene NGS is unable to fully resolve the CAG promoter sequence. Please refer to the depositor's sequence for this region of the plasmid.

Information for Cloning Grade DNA (Catalog # 11150-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here