CaM/pIRES2-eGFP
(Plasmid
#111499)
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PurposeElectrophysiology in HEK cell
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRES2-eGFP
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecalmodulin [Xenopus laevis]
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SpeciesX. laevis (frog)
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Entrez Genecalm2 (a.k.a. XELAEV_18026129mg, calm1, calm2-a, calm2-b, cam)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CaM/pIRES2-eGFP was a gift from Dan Minor (Addgene plasmid # 111499 ; http://n2t.net/addgene:111499 ; RRID:Addgene_111499) -
For your References section:
A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel Function. Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.. Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8. 10.1016/j.neuron.2018.01.035 PubMed 29429937
Map uploaded by the depositor.
