Kv7.5/pcDNA3.1
(Plasmid
#111498)
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PurposeElectrophysiology in HEK cell
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111498 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepotassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens]
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SpeciesH. sapiens (human)
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Entrez GeneKCNQ5 (a.k.a. Kv7.5, MRD46)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer GCGGACTTGAAGAAGTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid contains S296Y in KCNQ5. Mutation not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Kv7.5/pcDNA3.1 was a gift from Dan Minor (Addgene plasmid # 111498 ; http://n2t.net/addgene:111498 ; RRID:Addgene_111498) -
For your References section:
A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel Function. Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.. Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8. 10.1016/j.neuron.2018.01.035 PubMed 29429937