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Addgene

B1-4
(Plasmid #111466)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111466 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    translational elongation factor Tu
  • GenBank ID
    WP_000031783.1
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene comes from E.coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    B1-4 was a gift from Cheemeng Tan (Addgene plasmid # 111466 ; http://n2t.net/addgene:111466 ; RRID:Addgene_111466)

  • For your References section:

    Synthetic microbial consortia enable rapid assembly of pure translation machinery. Villarreal F, Contreras-Llano LE, Chavez M, Ding Y, Fan J, Pan T, Tan C. Nat Chem Biol. 2018 Jan;14(1):29-35. doi: 10.1038/nchembio.2514. Epub 2017 Nov 13. 10.1038/nchembio.2514 PubMed 29131146
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