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Addgene

Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1
(Plasmid #111461)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111461 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
  • Species
    H. sapiens (human)
  • Mutation
    Kv7.4 (1-645, Δ368-523)/I346D, C643A
  • Entrez Gene
    KCNQ4 (a.k.a. DFNA2, DFNA2A, KV7.4)
  • Tag / Fusion Protein
    • Ha Tag (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer GCGGACTTGAAGAAGTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1 was a gift from Dan Minor (Addgene plasmid # 111461 ; http://n2t.net/addgene:111461 ; RRID:Addgene_111461)

  • For your References section:

    A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel Function. Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.. Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8. 10.1016/j.neuron.2018.01.035 PubMed 29429937
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