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pCMV-Tag2-Sox9 T240A
(Plasmid #111457)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111457 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox9
  • Alt name
    SRY-box 9
  • Species
    H. sapiens (human)
  • Mutation
    T240A
  • Entrez Gene
    SOX9 (a.k.a. CMD1, CMPD1, SRA1, SRXX2, SRXY10)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for original WT SOX9 plasmid - https://www.ncbi.nlm.nih.gov/pubmed/?term=20651055
We used these primers for SDM Sox9 F7site5mut3F: 5'-ggcccaccgAccccacccAccgcccccaaaa-3' Sox9 F7site5mut3R: 5'-ttttgggggcggTgggtggggTcggtgggcc-3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag2-Sox9 T240A was a gift from Sharon Pine (Addgene plasmid # 111457 ; http://n2t.net/addgene:111457 ; RRID:Addgene_111457)

  • For your References section:

    SOX9 is targeted for proteasomal degradation by the E3 ligase FBW7 in response to DNA damage. Hong X, Liu W, Song R, Shah JJ, Feng X, Tsang CK, Morgan KM, Bunting SF, Inuzuka H, Zheng XF, Shen Z, Sabaawy HE, Liu L, Pine SR. Nucleic Acids Res. 2016 Oct 14;44(18):8855-8869. Epub 2016 Aug 26. 10.1093/nar/gkw748 PubMed 27566146
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