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Addgene

pV1386
(Plasmid #111445)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111445 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Backbone manufacturer
    RS Sikorski
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    URA3 ; clonNAT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaCas9/sgScADE2/stop-codon repair
  • gRNA/shRNA sequence
    sgScADE2/stop-codon repair
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pV1386 was a gift from Gerald Fink (Addgene plasmid # 111445 ; http://n2t.net/addgene:111445 ; RRID:Addgene_111445)

  • For your References section:

    New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi. Vyas VK, Bushkin GG, Bernstein DA, Getz MA, Sewastianik M, Barrasa MI, Bartel DP, Fink GR. mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25. 10.1128/mSphere.00154-18 PubMed 29695624
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