pV1326
(Plasmid
#111435)
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PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS416
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Backbone manufacturerRS Sikorski
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Vector typeYeast Expression, CRISPR
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Selectable markersURA3 ; clonNAT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaCas9/sgRNA-BsmBI stuffer
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gRNA/shRNA sequencesgRNA-BsmBI stuffer
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pV1326 was a gift from Gerald Fink (Addgene plasmid # 111435 ; http://n2t.net/addgene:111435 ; RRID:Addgene_111435) -
For your References section:
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi. Vyas VK, Bushkin GG, Bernstein DA, Getz MA, Sewastianik M, Barrasa MI, Bartel DP, Fink GR. mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25. 10.1128/mSphere.00154-18 PubMed 29695624
