pV1393
(Plasmid
#111430)
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PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepV1390
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Backbone manufacturerValmik Vyas
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Vector typeYeast Expression, CRISPR
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Selectable markersAmpicillin
Growth in Bacteria
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Bacterial Resistance(s)Nourseothricin (clonNat), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaCas9/sgRNA-BsmBI stuffer
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gRNA/shRNA sequencesgRNA-BsmBI stuffer
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pV1393 was a gift from Gerald Fink (Addgene plasmid # 111430 ; http://n2t.net/addgene:111430 ; RRID:Addgene_111430) -
For your References section:
New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi. Vyas VK, Bushkin GG, Bernstein DA, Getz MA, Sewastianik M, Barrasa MI, Bartel DP, Fink GR. mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25. 10.1128/mSphere.00154-18 PubMed 29695624