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Addgene

pV1081
(Plasmid #111425)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111425 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pV1025
  • Backbone manufacturer
    Valmik Vyas
  • Vector type
    Yeast Expression, CRISPR
  • Selectable markers
    clonNAT

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CaCas9/sgADE-NT2
  • gRNA/shRNA sequence
    caacaatcatacgacctaat
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pV1081 was a gift from Gerald Fink (Addgene plasmid # 111425 ; http://n2t.net/addgene:111425 ; RRID:Addgene_111425)

  • For your References section:

    A CRISPR system permits genetic engineering of essential genes and gene families. Vyas VK, Barrasa MI, Fink GR. Sci Adv. 2015;1(3):e1500248. 10.1126/sciadv.1500248 PubMed 25977940
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