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brr2-R1107M pRS313
(Plasmid #111417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS313
  • Backbone size w/o insert (bp) 4967
  • Total vector size (bp) 12000
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    BRR2
  • Alt name
    SLT22
  • Alt name
    PRP44
  • Alt name
    RSS1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    7000
  • Mutation
    R1107M aa mutation (note, not an RP allele)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    brr2-R1107M pRS313 was a gift from Christine Guthrie (Addgene plasmid # 111417 ; http://n2t.net/addgene:111417 ; RRID:Addgene_111417)

  • For your References section:

    Brr2 is a splicing fidelity factor. Mayerle M, Guthrie C. bioRxiv 2018. 354514 10.1101/354514
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