brr2-R1107A pRS313
(Plasmid
#111415)
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Purposebrr2-R1107A (endogenous promoter)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
- Backbone size w/o insert (bp) 4967
- Total vector size (bp) 12000
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBRR2
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Alt nameSLT22
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Alt namePRP44
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Alt nameRSS1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)7000
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MutationR1107A aa mutation
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
brr2-R1107A pRS313 was a gift from Christine Guthrie (Addgene plasmid # 111415 ; http://n2t.net/addgene:111415 ; RRID:Addgene_111415) -
For your References section:
Brr2 is a splicing fidelity factor. Mayerle M, Guthrie C. bioRxiv 2018. 354514 10.1101/354514