pScaf-5544.1
(Plasmid #111405)

• Purpose: pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)
• Depositing Lab: Shawn Douglas
• Publication: Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10.1093/synbio/ysy015. Epub 2018 Aug 9.

Backbone

• Vector backbone: pScaf
• Backbone size w/o insert (bp): 3154
• Total vector size (bp): 8299
• Modifications to backbone: Sequence inserted between KpnI/BamHI restriction sites
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: 37°C for plasmid growth, 30°C for phagemid ssDNA preparation (if transformed into HFR/F+/F' strain)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pScaf-GH'
• Insert Size (bp): 5163

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: TAAGGGATTTTGCCGATTTC
• 3′ sequencing primer: CACAGGAAACAGCTATGACCATGATT

Resource Information

• Supplemental Documents:
  • pScaf-5544.1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Preprint available at https://www.biorxiv.org/content/early/2018/04/27/309682