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Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT
(Plasmid #111400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens]
  • Species
    H. sapiens (human)
  • Mutation
    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A
  • Entrez Gene
    KCNQ4 (a.k.a. DFNA2, DFNA2A, KV7.4)
  • Promoter T7
  • Tags / Fusion Proteins
    • His Tag (N terminal on insert)
    • MBP tag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains L540A mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT was a gift from Dan Minor (Addgene plasmid # 111400 ; http://n2t.net/addgene:111400 ; RRID:Addgene_111400)

  • For your References section:

    A Calmodulin C-Lobe Ca(2+)-Dependent Switch Governs Kv7 Channel Function. Chang A, Abderemane-Ali F, Hura GL, Rossen ND, Gate RE, Minor DL Jr.. Neuron. 2018 Feb 21;97(4):836-852.e6. doi: 10.1016/j.neuron.2018.01.035. Epub 2018 Feb 8. 10.1016/j.neuron.2018.01.035 PubMed 29429937
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