pENTR U6-hTERTsh
(Plasmid #111385)

• Purpose: Knock down of hTERT transcript
• Depositing Lab: Klaus Holzmann
• Publication: Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046.

Backbone

• Vector backbone: pENTR/D-hH1
• Backbone manufacturer: Invitrogen
• Modifications to backbone: AV expressing shRNA against human telomerase reverse transcriptase (hTERT) was constructed from retroviral vector pMKO-1P-C3 kindly provided by William C. Hahn (Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA). In brief, an expression cassette for shRNA with U6 promoter targeting region 3114–3134 from the hTERT transcript was cloned into pENTR-hH1.
• Vector type: Gateway Entry Cloning

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: shRNA C3 against hTERT
• gRNA/shRNA sequence: TTTCATCAGCAAGTTTGGA
• Species: H. sapiens (human)
• Insert Size (bp): 329
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI/BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pENTR U6-hTERTsh.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry