pAV hTERTsh
(Plasmid
#111380)
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PurposeKnock down of hTERT transcript
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 111380 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAd/PL-DEST
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Backbone manufacturerInvitrogen
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Modifications to backbonepENTR U6-hTERTsh was transferred into Ad/PL-DEST for AV production as recommended by the manufacturer (Invitrogen).
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameshRNA C3 against hTERT
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gRNA/shRNA sequenceTTTCATCAGCAAGTTTGGA
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SpeciesH. sapiens (human)
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Insert Size (bp)399
- Promoter U6
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV hTERTsh was a gift from Klaus Holzmann (Addgene plasmid # 111380 ; http://n2t.net/addgene:111380 ; RRID:Addgene_111380) -
For your References section:
Telomere Transcripts Target Telomerase in Human Cancer Cells. Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K. Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046. 10.3390/genes7080046 PubMed 27537914