pLV UbC6-ARRET
(Plasmid #111378)

• Purpose: ARRET expression contains approximately 130 anti-sense telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
• Depositing Lab: Klaus Holzmann
• Publication: telomeric repeat-containing RNA (TERRA and ARRET) plasmids (unpublished)

Backbone

• Vector backbone: pLenti6-UbC/V5-DEST
• Backbone manufacturer: Invitrogen
• Modifications to backbone: The inserted fragments of the pENTR plasmids were transferred by Gateway recombination cloning technique (Invitrogen) to lentiviral (pLenti6/BLOCK-iT-DEST and pLenti4/V5-DEST) expression vectors.
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ARRET polyA
• Species: H. sapiens (human)
• Insert Size (bp): 868
• Promoter: UbC6

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pLV UbC6-ARRET.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry