pLV CMV-TERRA
(Plasmid #111375)

• Purpose: TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences
• Depositing Lab: Klaus Holzmann
• Publication: Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 10.3390/genes7080046.

Backbone

• Vector backbone: pLenti4/V5-DEST
• Backbone manufacturer: Invitrogen
• Modifications to backbone: The inserted fragments of the pENTR plasmids were transferred by Gateway recombination cloning technique (Invitrogen) to lentiviral (pLenti6/BLOCK-iT-DEST and pLenti4/V5-DEST) expression vectors.
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TERRA polyA
• Species: H. sapiens (human)
• Insert Size (bp): 868
• Promoter: CMV

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • pLV CMV-TERRA.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the Addgene Partial NGS Result for the verified backbone. Please note this result contains only a part (around 670 bps) of the 800 bps telomere sequence repeats, with further variant repeats not seen by Sanger sequencing by the Depositor.

This plasmid may be prone to recombination due to the telomere hexanucleotide repeats, and therefore screening multiple colonies for the correct insert length is recommended before proceeding with downstream work