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Addgene

PSuperPuroRetro-1660(hPot1)
(Plasmid #11135)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSuperPuroRetro
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNAi against human Protection of Telomeres 1
  • Alt name
    RNAi against hPOT1
  • Alt name
    protection of telomeres 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    65
  • GenBank ID
    NM_015450
  • Entrez Gene
    POT1 (a.k.a. CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hind III (not destroyed)
  • 3′ cloning site Bgl II (destroyed during cloning)
  • 5′ sequencing primer pLXSN5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target sequence is: GTACT AGAAG CCTAT CTCA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PSuperPuroRetro-1660(hPot1) was a gift from Christopher Counter (Addgene plasmid # 11135 ; http://n2t.net/addgene:11135 ; RRID:Addgene_11135)

  • For your References section:

    Loss of hPot1 function leads to telomere instability and a cut-like phenotype. Veldman T, Etheridge KT, Counter CM. Curr Biol. 2004 Dec 29. 14(24):2264-70. 10.1016/j.cub.2004.12.031 PubMed 15620654
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