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Addgene

puC19 preACT1(BrG)
(Plasmid #111326)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111326 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACT1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    branch site A to G mutation in intron

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

for use in in vitro splicing reactions

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    puC19 preACT1(BrG) was a gift from Christine Guthrie (Addgene plasmid # 111326 ; http://n2t.net/addgene:111326 ; RRID:Addgene_111326)

  • For your References section:

    The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing. Villa T, Guthrie C. Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305. 10.1101/gad.1336305 PubMed 16103217
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