pRS314 snu114-del(IV)
(Plasmid
#111321)
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Purposesnu114-del(IV)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 111321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS314
- Backbone size w/o insert (bp) 4783
- Total vector size (bp) 8000
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSNU114
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Alt nameGIN10
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Alt nameYKL173W
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3000
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Mutationdeletion of domain IVa
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS314 snu114-del(IV) was a gift from Christine Guthrie (Addgene plasmid # 111321 ; http://n2t.net/addgene:111321 ; RRID:Addgene_111321) -
For your References section:
Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Brenner TJ, Guthrie C. Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044. Epub 2005 May 23. 10.1534/genetics.105.042044 PubMed 15911574