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Addgene

PSUPER-1660s(hPot1)
(Plasmid #11132)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PSUPER
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNAi vector for human protection of telomeres 1(control for 1660)
  • Alt name
    RNAi vector for hPOT1(control for 1660)
  • Alt name
    hPOT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    65
  • Mutation
    N/A
  • Entrez Gene
    POT1 (a.k.a. CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)
  • 5′ sequencing primer M13R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains the 1660 sequence (GTACT AGAAG CCTAT CTCA), but is not a hairpin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PSUPER-1660s(hPot1) was a gift from Christopher Counter (Addgene plasmid # 11132 ; http://n2t.net/addgene:11132 ; RRID:Addgene_11132)

  • For your References section:

    Loss of hPot1 function leads to telomere instability and a cut-like phenotype. Veldman T, Etheridge KT, Counter CM. Curr Biol. 2004 Dec 29. 14(24):2264-70. 10.1016/j.cub.2004.12.031 PubMed 15620654
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