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pRS314 myc-SNU114
(Plasmid #111317)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111317 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone size w/o insert (bp) 4783
  • Total vector size (bp) 8000
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SNU114
  • Alt name
    GIN10
  • Alt name
    YKL173W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3000
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A single myc epitope was placed immediately after the start codon of SNU114 by inserting the annealed oligos oKD140 (5′-AATTCCCAGAACAAAAATTGATTTCTGAAGAAGATTTGAATA-3′) and oKD141 (5′-GATCTATTCAAATCTTCTTCAGAAATCAATTTTTGTTCTGGG-3′), which have overhanging EcoRI/BglII sites, into the same sites of pTB4. The resulting plasmid was named pTB19.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS314 myc-SNU114 was a gift from Christine Guthrie (Addgene plasmid # 111317 ; http://n2t.net/addgene:111317 ; RRID:Addgene_111317)

  • For your References section:

    Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Brenner TJ, Guthrie C. Genetics. 2005 Jul;170(3):1063-80. doi: 10.1534/genetics.105.042044. Epub 2005 May 23. 10.1534/genetics.105.042044 PubMed 15911574
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