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Addgene

pRS303 prp8-1
(Plasmid #111306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS303
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRP8
  • Alt name
    SLT21
  • Alt name
    RNA8
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    prp8-1 ( G1801D )
  • Entrez Gene
    PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note PRP8 has a G1801D mutation- unsure if this affects protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS303 prp8-1 was a gift from Christine Guthrie (Addgene plasmid # 111306 ; http://n2t.net/addgene:111306 ; RRID:Addgene_111306)

  • For your References section:

    Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3' splice site selection. Umen JG, Guthrie C. Genetics. 1996 Jun;143(2):723-39. PubMed 8725222
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