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pJU225 Prp8-TAP
(Plasmid #111305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111305 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS424
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRP8
  • Alt name
    RNA8
  • Alt name
    SLT21
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PRP8 (a.k.a. YHR165C, DBF3, DNA39, RNA8, SLT21, USA2)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid pJU225 (PRP8 TRPl2p) was constructed from
a previously described plasmid JDYl3 (GAL::PRP8) (BROWN
and BEGGS 1992). The galactose driven promoter in this plasmid
was removed by cutting with NheI and XhoI. A wild-type
copy of the promoter was amplified by PCR under nonmutagenic
conditions and used to replace the galactose driven
promoter. The entire PRP8 gene was then excised using XhoI
and NotI and ligated to plasmid pRS424 (TRPl 2p) also cut
with XhoI and NotI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJU225 Prp8-TAP was a gift from Christine Guthrie (Addgene plasmid # 111305 ; http://n2t.net/addgene:111305 ; RRID:Addgene_111305)

  • For your References section:

    Mutagenesis of the yeast gene PRP8 reveals domains governing the specificity and fidelity of 3' splice site selection. Umen JG, Guthrie C. Genetics. 1996 Jun;143(2):723-39. PubMed 8725222
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