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Addgene

yCp50-PRP5
(Plasmid #111303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    yCP50
  • Backbone size w/o insert (bp) 7950
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRP5
  • Alt name
    YBR237W
  • Alt name
    RNA5
  • Species
    S. cerevisiae (budding yeast)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full plasmid sequence contains several ambiguous bases in the backbone- Unsure if these regions will affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    yCp50-PRP5 was a gift from Christine Guthrie (Addgene plasmid # 111303 ; http://n2t.net/addgene:111303 ; RRID:Addgene_111303)

  • For your References section:

    Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Noble SM, Guthrie C. Genetics. 1996 May;143(1):67-80. PubMed 8722763
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