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Addgene

sub2-1 pRS315
(Plasmid #111301)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111301 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 6018
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SUB2
  • Alt name
    YDL084W
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    sub2-1

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional mutation in plasmid L206S- Not sure if this will affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sub2-1 pRS315 was a gift from Christine Guthrie (Addgene plasmid # 111301 ; http://n2t.net/addgene:111301 ; RRID:Addgene_111301)

  • For your References section:

    Deletion of MUD2, the yeast homolog of U2AF65, can bypass the requirement for sub2, an essential spliceosomal ATPase. Kistler AL, Guthrie C. Genes Dev. 2001 Jan 1;15(1):42-9. PubMed 11156604
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