pbabe-c-mycT58A+HRasG12V
(Plasmid #11130)

• Depositing Lab: Christopher Counter
• Publication: Kendall et al Cancer Res. 2005 Nov 1. 65(21):9824-8.

Backbone

• Vector backbone: pbabe
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: c-myc, HRas
• Alt name: c-myc
• Alt name: HRas
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 2300
• Mutation: c-myc T58A, A156T, and G438R; and H-Ras G12V
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Entrez Gene: Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI(c-myc), HindIII(H-Ras) (not destroyed)
• 3′ cloning site: EcoRI(c-myc), ClaI(H-Ras) (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains mouse c-myc and human Hras. This plasmid was generated by cloning c-MycT58A cDNA with flanking BamHI-EcoRI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site H-RasG12V cDNA in which HindIII/ClaI sites were again added by PCR.