HsCLYBL-FLAG
(Plasmid #111290)

• Purpose: mammalian expression
• Depositing Lab: Vamsi Mootha
• Publication: Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi: 10.1016/j.cell.2017.09.051. Epub 2017 Oct 19.

Backbone

• Vector backbone: pLys1
• Backbone size w/o insert (bp): 7500
• Total vector size (bp): 8500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: human CLYBL
• Species: H. sapiens (human)
• Insert Size (bp): 1026
• Entrez Gene: CLYBL (a.k.a. CLB)
• Tag / Fusion Protein:
  • linker and FLAG tag: TGGGSGGSGGSMDYKDDDDK (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe1 (not destroyed)
• 3′ cloning site: vector Xma1; insert Age1 (destroyed during cloning)
• 5′ sequencing primer: ATGGCGCTACGTCTGCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry