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Addgene

Prp40-pET21b
(Plasmid #111287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5442
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRP40
  • Alt name
    YKL012W
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • his6 (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Prp40-pET21b was a gift from Christine Guthrie (Addgene plasmid # 111287 ; http://n2t.net/addgene:111287 ; RRID:Addgene_111287)

  • For your References section:

    An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8. Price AM, Gornemann J, Guthrie C, Brow DA. RNA. 2014 Jan;20(1):46-60. doi: 10.1261/rna.041970.113. Epub 2013 Nov 14. 10.1261/rna.041970.113 PubMed 24231520
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