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Addgene

pHM4 (U6 in pSE360)
(Plasmid #111281)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111281 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSE360
  • Backbone manufacturer
    Stephen J. Elledge
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SNR6
  • Alt name
    U6
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    150

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sph1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer CAT TGC AAC AGT TTC AAC ACA GCC
  • 3′ sequencing primer GTG TAT CTT TAT CTT CCT GTA TGA TAT AGA TAA CTA AC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHM4 (U6 in pSE360) was a gift from Christine Guthrie (Addgene plasmid # 111281 ; http://n2t.net/addgene:111281 ; RRID:Addgene_111281)

  • For your References section:

    Multiple roles for U6 snRNA in the splicing pathway. Madhani HD, Bordonne R, Guthrie C. Genes Dev. 1990 Dec;4(12B):2264-77. PubMed 2149118
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