pbabe-hTERT+p53DD
(Plasmid #11128)

• Depositing Lab: Christopher Counter
• Publication: Kendall et al Cancer Res. 2005 Nov 1. 65(21):9824-8.

Backbone

• Vector backbone: pbabe
• Backbone size w/o insert (bp): 4500
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hTERT, p53 dominant negative
• Alt name: hTERT (human)
• Alt name: p53DD (mouse)
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 4600
• Mutation: p53 dominant negative (aa1-14; 303-390)
• Entrez Gene: TERT (a.k.a. CMM9, DKCA2, DKCB4, EST2, PFBMFT1, TCS1, TP2, TRT, hEST2, hTRT)
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1(hTERT) HindIII (p53DD) (not destroyed)
• 3′ cloning site: SalI(hTERT) Cla I(p53DD) (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated by cloning FLAG-hTERT cDNA with flanking EcoRI-SalI restriction sites added by PCR into the same sites of the polylinker of pBABEpuro or pBABEblast and by excising the selectable marker from the HindIII/ClaI sites and ligating into the same site p53DD cDNA in which HindIII/ClaI sites were again added by PCR.