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Addgene

HM110 prp16-2
(Plasmid #111277)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111277 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSE358
  • Backbone manufacturer
    Stephen J. Elledge
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 8300
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PRP16
  • Alt name
    PRP23
  • Alt name
    RNA16
  • Alt name
    YKR086W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3200
  • Mutation
    prp-2 (D575N)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (unknown if destroyed)
  • 3′ cloning site Sph1 (unknown if destroyed)
  • 5′ sequencing primer GCA GAT GAT AAA ACA CAA GAA GGC CC
  • 3′ sequencing primer CAT ATA TGA ATA TTT TGC CTA TTA GCA CGC TCT TCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HM110 prp16-2 was a gift from Christine Guthrie (Addgene plasmid # 111277 ; http://n2t.net/addgene:111277 ; RRID:Addgene_111277)

  • For your References section:

    Genetic interactions between the yeast RNA helicase homolog Prp16 and spliceosomal snRNAs identify candidate ligands for the Prp16 RNA-dependent ATPase. Madhani HD, Guthrie C. Genetics. 1994 Jul;137(3):677-87. PubMed 8088513
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