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pET-30a(+)-Syk-tSH2-FX
(Plasmid #111272)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111272 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-30a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 5943
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), with interdomain A (Phe 119 to His 162) substituted by a flexible 20aa linker
  • Alt name
    Spleen tyrosine kinase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    714
  • Mutation
    interdomain A (Phe 119 to His 162) substituted by a flexible 20-amino-acid linker (GGS)3GS(GGS)3
  • GenBank ID
    NP_035648
  • Entrez Gene
    Syk (a.k.a. Sykb)
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (unknown if destroyed)
  • 3′ cloning site Xho I (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

encoding the following protein sequence: MSANHLTYFFGNITREEAEDYLVQGGMTDGLYLLRQSRNYLGGFALSVAHNRKAHHYTIERELNGTYAISGGRAHASPADLCHYHSQEPDGLICLLKKPFNRPPGVQPKTGPGGSGGSGGSGSGGSGGSGGSEKMPWFHGNISRDESEQTVLIGSKTNGKFLIRARDNSGSYALCLLHEGKVLHYRIDRDKTGKLSIPEGKKFDTLWQLVEHYSYKPDGLLRVLTVPCQKIGAQ

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-30a(+)-Syk-tSH2-FX was a gift from Carol Post (Addgene plasmid # 111272 ; http://n2t.net/addgene:111272 ; RRID:Addgene_111272)

  • For your References section:

    Insights into the allosteric regulation of Syk association with receptor ITAM, a multi-state equilibrium. Feng C, Post CB. Phys Chem Chem Phys. 2016 Feb 17;18(8):5807-18. doi: 10.1039/c5cp05417f. 10.1039/c5cp05417f PubMed 26468009
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